The two photographs show different kinds of lady bugs, in the shot of two insects the one on the right is a young lady bug (an “instar” e.g. an earlier stage of growth). There is a world to learn about when it comes to entomology. You can join Bugguide.net to help with identification and if you send them your photos of insects you cannot identify, the entomologists will help. There are some other great guides to insects.Arthur V. Evans, Field Guide to Insects and Spiders of North America was my first guide and still a wonderful help.
Macro: There are two things in short supply in close-up and macro photography, depth of field and light. The closer we get to something, the narrower the depth of field at any given F stop and the less light reflected off our subject. If you get close enough into the true 1:1 macro range, depth of field becomes wafer thin even at F 16. If extreme macro photography is your thing then I suggest this site: http://extreme-macro.co.uk.
If we stand a little further back and are prepared to crop images like many if not most pros do, then we can avoid extreme solutions. Some say its best to shoot insects side on, that is supposed to get you maximum depth of field but is not the most practical solution. Moving back and cropping in post production is, the use of extension tubes is (see yesterday’s post). The point is to be prepared for the lack of depth of field. Using F stops above F 14 can lead to some softness (diffraction of which more in another post). There is a lot of science behind the comments here but I will spare you. Next I will propose solutions for the lighting problem.
In an earlier post I mentioned that aperture should be multiplied by the crop factor, and that as a result crop factor cameras give some advantage in macro photography. Mike Simms commented on this issue: “but the speed/light gathering properties of the aperture remain the same. In other words if your settings on a full-frame camera were say F2.8 at 1/2000sec at ISO 100, that does not mean that the exposure would become an F5.6 at 1/1000thsec at ISO 100 on an M43 sensor with its x2 crop factor. On the M43 your exposure settings would be identical to the full frame camera, only the depth of field would act like F5.6.”